参考文献3中记载了通过多酶级联系统，以蔗糖和GlcNAc为底物，在蔗糖

Reference 3 describes that through a multi-enzyme cascade system, using sucrose and GlcNAc as substrates, sucrose phosphorylase, UDP-glucose hexose-1-phosphate uridylyl transferase, and UDP glucose 4-epimerase The reaction lasted for 600 hours under the catalysis of four enzymes, lactose-N-biological phosphorylase, and the yield of synthesized LNB was 83%. The long reaction time of this process is not conducive to industrial production.

Reference 3 records the synthesis of LNB through a multi enzyme cascade system using sucrose and GlcNAc as substrates, catalyzed by four enzymes: sucrose phosphorylase, UDP glucose hexose 1 phosphate uridine transferase, UDP glucose 4 epimerase, and lactose N biophosphatase for 600 hours. The yield of LNB synthesis is 83%. The characteristic of long reaction time in this process is not conducive to industrial production.

Reference 3 records that through a multi-enzyme cascade system, sucrose and GlcNAc were used as substrates and reacted for 600 hours under the catalysis of sucrose phosphorylase, UDP- hexose -1- phosphate uridine transferase, UDP-glucose 4- epimerase and lactose -N- biological phosphorylase, and the yield of LNB was 83%. The reaction time of this process is long, which is not conducive to industrial production.