The Folin–Ciocalteu reagent was use

The Folin–Ciocalteu reagent was used to determine the total phenol content of Asian pear juice powder (Eghdami and Sadeghi 2010). First, 9 mL of 80% methanol solution was mixed with 1 g of Asian pear juice powder at 40 C for 24 h and cooled at room temperature. The solution was centrifuged at 2016 RCF (g) for 15 min and then filtered through filter paper. A volume of 200 lL of sample extract, 800 lL of Folin–Ciocalteu reagent and 2 mL of 7.5% sodium carbonate were incorporated and distilled water added for a total volume of 7 mL. The solution was kept in the dark for 1 h. The absorbance at 765 nm of the sample mixture was measuredusing a UV spectrophotometer (Optizen 2120UV, Mecasys, Seoul, Korea). Gallic acid was used as standard and expressed as mg gallic acid equivalents (GAE)/100 g.