The addition of mixed wine spirit to brandy is easy way to adulterate brandy. To avoid the misleading ofthe consumers, it is necessary to develop reliable method to detect adulteration of brandy. In this workexcitation emission matrix fluorescence in combination with parallel factor analysis (PARAFAC) and partial least squares (PLS) regression was used to determine the content of mixed wine spirit in adulteratedbrandy samples. Excitation emission matrix fluorescence spectra were measured in the emission wavelength range of 485–580 nm and in the excitation wavelength range of 363–475 nm. The model createdusing PARAFAC–PLS was able to predict the mixed wine spirit level in adulterated brandy with the rootmean square error of prediction (RMSEP) value of 1.9% and a square of the correlation (R2) between thereference contents and the predicted values of 0.995