TUNEL检测细胞凋亡离心重悬各组细胞, 调整细胞浓度为1×108 L

TUNEL detects cell apoptosis by <br>centrifugation and resuspends the cells in each group, adjusts the cell concentration to 1×108 L-1, inoculates the cells in a 3.5 cm culture dish with a built-in cover glass, and incubates in a 37 ℃ incubator for 24 h. Replace with fresh medium and incubate for 72 h. Observe the cell morphology, take pictures, take out the cover glass, use the TUNEL kit, operate according to the kit instructions, add fluorescein-labeled deoxynucleotide mixed working solution, incubate at 37 ℃ for 1 h, use 493 nm under a fluorescence microscope Blue light observation, count the number of apoptotic cells in each high-power field, and randomly count 5 fields for each sample. After the observation, proceed to DAB color development and mount the film.

TUNEL detects apoptosis.<br>Centrifugation re-suspends each group of cells, adjusts the cell concentration to 1 x 108 L-1, inoculation cells in a 3.5 cm Petri dish with built-in cover slides, 37 degrees C temperature box incubation 24 h replacement fresh media, incubation 72 h, observation of cell morphology, 2 photogram, take out cover slides, use TUNEL kit, according to the kit instruction manual operation, add lucifer-labeled DNA mixed working fluid, 37 degrees C incubation 1 h, under fluorescent microscope with 493 nm blue light observation, calculate the number of apoptosis in each high field of view, Each sample randomly counts 5 field of view. After observation continues DAB coloring, sealing.

TUNEL检测细胞凋亡离心重悬各组细胞, 调整细胞浓度为1×108 L-1, 接种细胞于内置盖玻片的3.5 cm培养皿, 37 ℃温箱孵育24 h更换新鲜培养基, 孵育72 h, 观察细胞形态、照相, 取出盖玻片, 采用TUNEL试剂盒, 按试剂盒说明书操作, 加入荧光素标记的脱氧核苷酸混合工作液, 37 ℃孵育1 h, 在荧光显微镜下用493 nm蓝光观察, 计算每个高倍视野下凋亡细胞数, 每一样本随机计数5个视野。观察完毕继续进行DAB显色, 封片。<br>