针对宁夏灌区盐碱地农田土壤氮素转化微生物过程不清和氮肥利用较低等问题，

In view of the problems of unclear nitrogen conversion microbial processes and low nitrogen fertilizer utilization in saline-alkali farmland soils in Ningxia irrigation areas, 15N stable isotope, qPCR and other molecular biological methods were used to carry out key microbial process and nitrogen utilization studies on nitrogen conversion in saline-alkali farmland soils The impact of different farmland management measures on saline-alkali farmland nutrient management and exogenous carbon / nitrogen input on the microbial process of nitrogen conversion in saline-alkali soils, analysis of the composition and distribution characteristics of key microbial community in nitrogen conversion and its relationship with environmental factors, and analytical participation The contribution and function of soil nitrogen transformation microbial functional genes in the process of nitrogen transformation, clarify the main control factors of the nitrogen transformation function microbial genes in saline-alkali soils and their response to carbon and nitrogen input in saline-alkali soils, and reveal the nitrogen of saline-alkali farmland soil ecosystem The microbial driving mechanism of element conversion provides a theoretical basis for optimizing the effective management of nitrogen in saline-alkali farmland and the efficient use of nitrogen fertilizer. Published 4-6 high-quality academic papers through project research, and cultivated a research team with higher research level and innovative ability to improve soil nutrients and microbial regulation in saline soil.

In view of the problems such as the unclear process of nitrogen conversion microorganism and the low utilization of nitrogen fertilizer in the soil of the saline field in Ningxia irrigation area, the molecular biology methods such as 15N stable isotope and qPCR were used to carry out the research on the key microbial process of nitrogen conversion and nitrogen utilization of soil in the saline farmland, and to find out the influence of different farmland management measures such as agricultural nutrient management, external carbon/nitrogen input and so on the microbial transformation of nitrogen in saline soil. To transform the composition, distribution characteristics and the connection between the key microbial community and its relationship with the environmental factors, to analyze the contribution and the size of the nitrogen transformation gene involved in soil nitrogen transformation, to clarify the main control factors of the microbial genes of the nitrogen conversion function of saline and its response to the carbon-nitrogen input in the saline-alkali land, to reveal the microbial driving mechanism of the soil nitrogen transformation in the soil soil of saline soil, and to provide a theoretical basis for optimizing the effective management and efficient utilization of nitrogen nitrogen in the agricultural area. Through the project research published 4-6 high-quality academic papers, training a high level of research and innovation of the soil nutrient upgrading and microbial regulation of the research team.

In order to solve the problems of unclear microbial process of nitrogen transformation and low nitrogen utilization in saline alkali soil of Ningxia irrigation area, 15N stable isotope and qPCR were used The key microbial processes of nitrogen transformation and nitrogen utilization in saline alkali soil were studied by using molecular biological methods. The effects of different management measures such as nutrient management and exogenous carbon / nitrogen input on the microbial processes of nitrogen transformation in saline alkali soil were found out. The composition and distribution characteristics of the key microbial communities of nitrogen transformation and their relationship with environmental factors were analyzed The contribution and role of microbial functional genes involved in soil nitrogen transformation in the process of nitrogen transformation, the main control factors of microbial functional genes involved in soil nitrogen transformation and their response to carbon and nitrogen input in saline alkali soil were clarified, the microbial driving mechanism of nitrogen transformation in saline alkali soil soil ecosystem was revealed, which could improve the effective management and efficient utilization of nitrogen in saline alkali soil Provide theoretical basis. Through the project research, 4-6 high-quality academic papers were published, and a research team with high research level and innovation ability for Soil Nutrient Improvement and microbial regulation in saline alkali land was cultivated.<br>