Place it in a 5% CO, 37 ° C, satura

Place it in a 5% CO, 37 ° C, saturated humidity incubator for 4 hours, discard the culture solution, add 90uL of simple culture solution to each well, and add 10uLMTT (5mg / mL) solution. Incubate in the incubator for 4 hours, discard the culture medium, and add 150uL of dimethylsulfoxide (DMEM) solution to each well. Quickly shake with a plate shaker for 15min. After the blue-violet crystal formazan in each well is completely dissolved, use a microplate reader to detect the light absorption value (OD value) at a wavelength of 570nm. (4) Detection of the effects of PEP and PEP1 on the proliferation of A549 and 93T cells by MTT method. A549 and 93T cells growing in logarithmic phase were trypsinized into single cells, and fresh DME / F-1 complete culture solution (DMEMHighGlucose culture Solution) Stop digestion, centrifuge (1500r / min, 5min at room temperature), discard the supernatant, add complete culture, pipette into a single cell suspension, and inoculate at 96 × 1 cell density in 96 cells per mL. In a well plate, the inoculation volume of each well is 100uL, and place it in a 5% CO, 37 ° C, saturated humidity incubator for 4 hours, discard the culture solution,