对VEGFR-Fc融合蛋白SDS-聚丙烯酰胺凝胶电泳(简称SDS—PA

对VEGFR-Fc融合蛋白SDS-聚丙烯酰胺凝胶电泳(简称SDS—PAGE)（纯度+分子量）分析方法进行验证，以确认该分析方法可以准确检测样品中VEGFR-Fc融合蛋白的纯度及其分子量。用SDS-聚丙烯酰胺凝胶电泳在专属性、精密度、耐用性进行验证。经过SDS-聚丙烯酰胺凝胶电泳。方法学验证结果为：专属性为空白溶液（辅液）检测无明显干扰；光照实验样品，检测聚体有明显变化；精密度为重复性为非还原电泳纯度RSD为0.12%，还原电泳纯度RSD为0.62%，分子量RSD为0.77%；中间精密度：不同人员间非还原电泳纯度RSD为0.62%，还原电泳纯度RSD为0.75%，分子量RSD为1.21%；耐用性为使用3个不同批次预制胶非还原电泳纯度RSD为0.78%，还原电泳纯度RSD为0.42%，分子量RSD为0.81%。根据以上验证结果证明SDS-聚丙烯酰胺凝胶电泳（考染）方法在专属性、精密度和耐用性等方面均符合要求，因此，该方法适用于样品中VEGFR-Fc融合蛋白纯度和分子量的检测。

对VEGFR-Fc融合蛋白SDS-聚丙烯酰胺凝胶电泳(简称SDS—PAGE)（纯度+分子量）分析方法进行验证，以确认该分析方法可以准确检测样品中VEGFR-Fc融合蛋白的纯度及其分子量。 用SDS-聚丙烯酰胺凝胶电泳在专属性、精密度、耐用性进行验证。 经过SDS-聚丙烯酰胺凝胶电泳。 方法学验证结果为：专属性为空白溶液（辅液）检测无明显干扰；光照实验样品，检测聚体有明显变化；精密度为重复性为非还原电泳纯度RSD为0.12%，还原电泳纯度RSD为0.62%，分子量RSD为0.77%；中间精密度：不同人员间非还原电泳纯度RSD为0.62%，还原电泳纯度RSD为0.75%，分子量RSD为1.21% ；耐用性为使用3个不同批次预制胶非还原电泳纯度RSD为0.78%，还原电泳纯度RSD为0.42%，分子量RSD为0.81%。 根据以上验证结果证明SDS-聚丙烯酰胺凝胶电泳（考染）方法在专属性、精密度和耐用性等方面均符合要求，因此，该方法适用于样品中VEGFR-Fc融合蛋白纯度和分子量的检测。 ...

The VEGFR-Fc fusion protein SDS- polyacrylamide gel electrophoresis (SDS PAGE) (purity + molecular weight) analysis method was validated to confirm that the method can accurately detect the purity and molecular weight of VEGFR-Fc fusion protein in the sample. The specificity, precision and durability were verified by SDS- polyacrylamide gel electrophoresis. SDS- polyacrylamide gel electrophoresis. The results of methodology validation were as follows: specificity: blank solution (auxiliary solution) detection had no obvious interference; light test sample, detection of polymer had obvious changes; precision: RSD of non reduction electrophoresis purity was 0.12%, RSD of reduction electrophoresis purity was 0.62%, RSD of molecular weight was 0.77%; intermediate precision: RSD of non reduction electrophoresis purity was 0.62%, RSD of reduction electrophoresis purity was 0.75% The relative standard deviation (RSD) of molecular weight was 1.21%, the RSD of non reduction electrophoresis was 0.78%, the RSD of reducing electrophoresis was 0.42%, and the RSD of molecular weight was 0.81%. According to the above verification results, the SDS- polyacrylamide gel electrophoresis method is suitable for specificity, precision and durability. Therefore, the method is suitable for the detection of the purity and molecular weight of VEGFR-Fc fusion protein in samples.<br>