Separately inject equal volumes of

Separately inject equal volumes of derivatized Blank, derivatized System suitability solution, and derivatized Sample solution into thechromatograph and record the chromatograms. Identify the two diastereomer peaks due to (+)-FLEC derivatives of D-carnitine and L-carnitine in the chromatograms of the derivatized System suitability solution and derivatized Sample solution. Depending on thepurity of the derivatizing reagent, these two peaks may contain co-eluting enantiomers of (−)-FLEC derivatives withD- and L-carnitines, which are accounted in the percentage calculations below. There should be no peaks observed at the retention timesof D- and L-carnitine derivatives in the chromatogram of the derivatized Blank.