Amplification was performed in a 20-

Amplification was performed in a 20-ll reaction con-taining the genomic DNA on one 1.2-mm punch of FTApaper, 0.25 l M of each primer, 150 l M of each d NTP(Eppendorf, Hamburg, Germany), 2.5 mM of Mg2?, 0.5 Uof Taq DNA polymerase (Qiagen, Hilden, Germany) and19 reaction buffer supplied. The thermal profile consistedof 2 min at 94°C, followed by 35 cycles of 30 s at 94°C,30 s at 57°C and 50 s at 72°C, with a final extension of5 min at 72°C. Amplification was carried out in an iCycler(Bio-Rad, Hercules, CA, USA).