[Objective]The aim of this study was to study the growth and development characteristics of Taxus yunnanensis and the annual change of taxol content. [Method]Lijiang area was selected for the field experiment. The annual change of growth character and taxol content of the same provenance,age and substrate of seedlings of T. yunnanensis were studied,which were cultured in the open. [Result]The prosperity period of height growth of the seedlings of T. yunnanensis was May to August. There was the excentric crown phenomenon on the seedlings of T. yunnanensis. The maximum taxol content of the seedlings of T. yunnanensis appeared during June to July. [Conclusion]This study provided reference for seedling management,adaptation strategy,resource protection and industrialization of T. yunnanensis.