Immerse 10mg of nanofiber membrane in 10ml of PBS solution (pH=7.4) and incubate it in a 37 ℃, 50rpm constant temperature oscillator (Taichang, Jiangsu) to test the release characteristics of ASTA. At a specific time point, take 1ml of solution and add an equal amount of fresh buffer to continue incubating to ensure a constant amount of PBS buffer during the re release process. According to the standard curve (y=0.09235x-0.00257, R2=0.9983), the release amount of astaxanthin in the culture medium was measured at 489nm with an ultraviolet spectrophotometer (UV – vis universal analysis TU-1901). Each sample is tested three times in parallel.
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