stabilize the NaDPH oxidase 2 (NOX2) complex to produce reactive oxygen species (rOs)22,149. Both Ptdins(3,4,5)P3 and rOs are necessary for conjugating autophagy-related protein LC3-ii to lipidsat the phagosomal membrane, a process termed ‘LC3-associated phagocytosis’. LC3-associated phagocytosis promotes phagolysosomal assembly and acidification and thereby facilitates aC degradation52. NOX2 deficiency in chronic granulomatous disease has been associated with impaired efferocytosis