对sod进行RT-qPCR，结果如图10所示，三羧酸循环相关酶调控基因

RT-qPCR was performed on sod, and the results are shown in Figure 10. The sdhA and ace genes regulating the citric acid cycle-related enzymes all showed significant up-regulation, and the expression of multi-copper oxidase regulating genes was significantly inhibited. At 24 h of the reaction, The tryptophan synthase regulatory gene trpE also showed a significant down-regulation. This is consistent with the transcriptome results. It shows that the iron oxide produced during the Fe2+ oxidation inhibits electron transfer and material transport, inhibits the synthesis of copper oxidase, and inhibits the manganese biological oxidation process of MnB1.

RT-qPCR was carried out on sod, and the results showed in Figure 10 that the triacetic acid circulatory enzyme regulatory genes sdhA and ace showed significant upward adjustment, the expression of polycoaminase regulatory gene was significantly inhibited, and at 24 h of the reaction, the tryptophan synthase regulatory gene trpE also showed significant downward adjustment. This is consistent with the transcription group results. It is explained that the iron oxide produced in the process of Fe2 plus oxidation inhibits electron transmission and material transport, inhibits the synthesis of poly-copper oxidase, and then inhibits the process of manganese biooxidation of MnB1.

RT-qPCR was performed on SOD. The results showed that SDH and ace of the three carboxylic acid cycle related enzyme regulatory genes showed significant up regulation, and the expression of the regulatory gene of PCE was significantly inhibited. At 24 hours of reaction, trpe, the tryptophan synthetase regulatory gene, also showed a significant down regulation. This is consistent with the transcriptome results. The results show that the iron oxide formed in the oxidation process of Fe2 + inhibits the electron transfer and material transport, inhibits the synthesis of copper oxidase, and further inhibits the manganese biological oxidation process of mnb1.<br>