() MTT colorimetric method to detect cell survival rate [76] MTT (3- (4,5-dimethylthiazole-)-, 5-diphenyltetrazolium bromide) colorimetric method is a method for detecting cells Methods of survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystal formazan (Formazan) and deposit in cells, while dead cells do not have this function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at 570nm with a microplate reader, which can indirectly reflect the number of living cells. Within a certain number of cells, the amount of MTT crystals formed is directly proportional to the number of living cells. This method has been widely used in the detection of some biologically active factors, large-scale anti-tumor drug screening, cytotoxicity tests, and tumor radiosensitivity determination. It is characterized by high sensitivity and economy.