Lipids have been drawing more and more attention due to their vitalroles in numerous cellular functions and biological processes,including energy storage, signal transduction, cell adhesion andmigration, metabolism, etc.1 Previous studies have shown that manydiseases, such as diabetes, obesity, Alzheimer’s disease, and neurodegenerative diseases, are closely correlated with abnormal lipidmetabolism.2,3 Thus, the comprehensive in situ analysis of lipids inbiological tissues is an important aspect of lipidomics.