FIGURE 9.18 Targeted library preparation for NGS using probe enrichment. Fragmented DNA is denatured and hybridized to region-specific biotinylated probes. The probes are bead-captured, and the hybridized regions are amplifi ed for sequencing. Probes may be short oligomers that can be extended across the region to be sequenced. The selected regions can then be amplified with tailed primers to add bar codes and sequencingprimer-bindingsites.