In order to further determine whether the adsorbed metal ions or the carbon particles adsorbed on the metal ions induced the cellular inflammatory response, we found that the same dose of metal ions Al (0.5 μg/ml), Cd (0.005 μg/ml), Cu (0.25μg/ml), Zn (0.5μg/ml) exposed the cells alone without significant changes in il6 and il8 protein levels.
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