Light is the most direct factor influencing photosynthesis. This study demonstrates the effects of different light quality on photosynthetic performance in tomato leaves and aims to improve environmental control during crop growth. Our results suggest that different light quality treatments have significant effects on various photosyntheticrelated processes. The white light and the combination of red and blue light exhibited higher photosynthetic operation efficiency in tomato, while the blue and purple light irradiation significantly reduced the photosynthetic efficiency via influencing plant morphology, damaging the thylakoid membrane structure, reducing the photosynthetic pigment content and photosynthetic electron transport rate. However, CEF was enhanced to induce NPQ under the blue and purple light treatment. We posit that this mainly function to protect PSII and PSI from photoinhibition via lumen acidification. Understanding the effects of wavelength-specific lighting on photosynthetic electron transport activity in tomato leaves has significant implications on basic academic research as well as commercial greenhouse production. The underlying molecular mechanism remains unknown and further study is necessary to fully understanding these phenomena to improve the yield and quality of crops.