山东菏泽某济宁青山羊养殖场为确诊发生脑膜脑炎和急性败血病的济宁青山羊病

山东菏泽某济宁青山羊养殖场为确诊发生脑膜脑炎和急性败血病的济宁青山羊病例，解剖并采集病死济宁青山羊的脑、肝脏、肾脏作为病料，对病原菌进行了分离、鉴定，并进行了药敏试验，革兰染色，生化试验，运动性试验，毒力试验。检测结果显示，该分离菌的生物化学特征、培养特征、溶血性特征等均与李氏杆菌的病原学特征相一致，证实该李氏杆菌感染，并对阿奇霉素、头孢噻肟钠和丁胺卡那霉素具有较高的敏感性，本实验为养殖户提供养殖技术参考。

A Jining Qingshan Sheep Farm in Heze, Shandong Province was established to diagnose meningoencephalitis and acute sepsis in Jining Qingshan Sheep. The brain, liver, and kidneys of the deceased Jining Qingshan Sheep were dissected and collected as diseased materials. The pathogenic bacteria were isolated and identified, and drug sensitivity tests, Gram staining, biochemical tests, exercise tests, and toxicity tests were conducted. The test results showed that the biochemical, cultural, and hemolytic characteristics of the isolated bacteria were consistent with the pathogenic characteristics of Listeria, confirming that the Listeria was infected and had high sensitivity to azithromycin, cefotaxime sodium, and amikacin. This experiment provides breeding technology reference for farmers.

In order to diagnose the cases of meningoencephalitis and acute septicemia in Jining Qingshan sheep farm in Heze, Shandong Province, the brain, liver and kidney of the dead Jining Qingshan sheep were dissected and collected as diseased materials, and the pathogenic bacteria were isolated and identified, and the drug sensitivity test, Gram staining, biochemical test, exercise test and toxicity test were carried out. The results showed that the biochemical characteristics, culture characteristics and hemolytic characteristics of the isolated strain were consistent with the pathogenic characteristics of Listeria, which confirmed that Listeria was infected and had high sensitivity to azithromycin, cefotaxime sodium and amikacin. This experiment provided breeding technical reference for farmers.