用同样的方法将pBM101转化入大肠杆菌DH5α中，经酶切及测序验证后

The same method was used to transform pBM101 into E. coli DH5α. After digestion and sequencing verification, the extracted plasmid was transformed into E. coli BL21, and the positive transformants were selected on LB plates containing 100 μg/mL AMP to obtain the recombinant strain BM101

Using the same method to convert pBM101 into E. coli DH5 alpha, after enzymatic cutting and sequencing verification, extract the plasmid into E. coli BL21, in the 100 sg/mL AMP-positive test conversion, that is, the recombinant strain BM101

Pbm101 was transformed into E.coli DH5 α by the same method. After enzyme digestion and sequencing, the plasmid was extracted and transformed into E.coli BL21. The positive transformants were screened on the LB plate containing 100 μ g / ml amp<br>