In this paper, the effects of the different humidity and operating temperature on the PEM fuel cell performance are investigated by numerical and experimental methods. More importantly, the innovation of this paper is to study the performance of fuel cell from the perspective of temperature heat transfer by asymmetric humidification. In the simulation, a novel temperature and heat transfer (T&HT) model is presented based on the temperature gradient phenomenon and thermal transfer in the stack. Furthermore, two different forms of thermal transfer are considered for thermal conduction and thermal convection. In the experiment, the Taguchi method is adopted in the orthogonal experiment in a self-developed PEM fuel cell test stand. First of all, symmetrical humidification experiments were carried out with three different operating temperatures. After that, the asymmetric humidification experiment was put into practice with 60℃ operating temperature. Furthermore, the polarization curves are obtained by experiment and simulation results and were compared and analyzed.