Pathological tissue slicing technology is a commonly used auxiliary detection technique, commonly used for pathological diagnosis. This experiment used three mouse models of cerebral hemorrhage, unilateral ureteral ligation, and myocardial infarction to collect and fix specimens of brain tissue, kidney tissue, and heart tissue after spinal dislocation. Subsequently, the fixed specimens were subjected to experimental processing according to the process of dehydration, embedding, slicing, staining, and microscopic examination. The quality of the paraffin blocks obtained from embedding, the quality of the slices, and the final staining microscopic examination results were analyzed. Based on the comprehensive analysis, it is necessary to make high-quality paraffin sections that meet the requirements of direct fixation after tissue killing and extraction, with a tissue sampling thickness of 2mm, embedding after complete tissue dehydration, and a slice thickness of 7 μ m. The dyeing time should be accurately controlled according to the experimental preset process and other requirements. This experiment provides a reference for improving the level of pathological tissue slicing technology.
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