病理组织切片技术是一种辅助常用检测技术，常用于病理诊断。本实验利用脑出

病理组织切片技术是一种辅助常用检测技术，常用于病理诊断。本实验利用脑出血、单侧输尿管节扎、心肌梗死三种小鼠模型，通过脊椎脱臼法致死后对脑部组织、肾脏组织和心脏组织进行标本采集及固定。随后将固定的标本按照脱水、包埋、切片、染色、镜检流程进行实验处理，通过对包埋所得石蜡块质量好坏、切片质量好坏及最终染色镜检结果分析。综合得出做出优质的石蜡切片需要满足组织现杀现取后直接固定、组织取材厚度在2mm，组织脱水完全后进行包埋、切片厚度7μm、染色时间按实验预设流程精准控制等要求。该实验为提高病理组织切片技术水平提供参考。

Pathological tissue slicing technology is a commonly used auxiliary detection technique, commonly used for pathological diagnosis. This experiment used three mouse models of cerebral hemorrhage, unilateral ureteral ligation, and myocardial infarction to collect and fix specimens of brain tissue, kidney tissue, and heart tissue after spinal dislocation. Subsequently, the fixed specimens were subjected to experimental processing according to the process of dehydration, embedding, slicing, staining, and microscopic examination. The quality of the paraffin blocks obtained from embedding, the quality of the slices, and the final staining microscopic examination results were analyzed. Based on the comprehensive analysis, it is necessary to make high-quality paraffin sections that meet the requirements of direct fixation after tissue killing and extraction, with a tissue sampling thickness of 2mm, embedding after complete tissue dehydration, and a slice thickness of 7 μ m. The dyeing time should be accurately controlled according to the experimental preset process and other requirements. This experiment provides a reference for improving the level of pathological tissue slicing technology.

Pathological tissue section technique is an auxiliary common detection technique, which is often used for pathological diagnosis. In this experiment, three mouse models of cerebral hemorrhage, unilateral ureteral ligation and myocardial infarction were used to collect and fix samples of brain tissue, kidney tissue and heart tissue after death by spinal dislocation. Subsequently, the fixed specimens were treated by dehydration, embedding, slicing, staining and microscopic examination, and the quality of paraffin blocks obtained by embedding, the quality of slices and the final staining microscopic examination results were analyzed. It is concluded that to make high-quality paraffin sections, it is necessary to meet the requirements of direct fixation after tissue is killed and removed, the thickness of tissue is 2mm, embedding after tissue dehydration is complete, the thickness of sections is 7μm, and the dyeing time is accurately controlled according to the experimental preset process. This experiment provides reference for improving the technical level of pathological tissue sectioning.