Figure 1. CF®568 (left) produces better images than Cy®3b (right) in 3-D STORM microscopy. Fixed cells were stained with mouse anti-tubulin antibody followed by dye-conjugated antimouse secondary antibodies. See Figure 2 legend for imaging conditions. Dye molecules were photoswitched and imaged using a 560 nm laser; a 405 nm laser was used to assist dye reactivation to the emitting state.