采集0～20cm耕层土壤，去除植物残根和石块等杂物后按四分法将同一小区

Collect 0-20 cm ploughed soil, remove plant roots and stones and other debris, and then divide the soil of the same plot sampling point into 2 parts according to the quartile method. 1 part is air-dried and sieved to determine the soil chemical properties. One portion was stored in a sterile bag of dry ice and brought back to the laboratory. It was stored in a refrigerator at -80 ℃ for soil DNA genome extraction. The basic physical and chemical properties of the tested soil after the end of the growing season are shown in Table 2

Collect0 to 20 cm tillage soil, remove plant residues and stones and other debris according to the four-point method of the same area sampling point soil equivalent mixed into 2 parts, 1 air-drying sieve used to determine soil chemical properties, 1 loaded into a sterile bag dry ice preservation and brought back to the laboratory, -80 degrees C refrigerator preservation for soil DNA genome extraction. See Table 2 on the basic physical and chemical properties of the soil for testing after the end of the growth season

Collect 0-20cm arable soil, remove plant residual roots, stones and other debris, and then divide the soil of the same sampling point into 2 parts according to the quartering method. One part is dried and screened to determine the chemical properties of the soil, and the other part is put into a sterile bag of dry ice for preservation and then brought back to the laboratory. The - 80 ℃ refrigerator is used for soil DNA genome extraction. See Table 2 for the basic physical and chemical properties of the tested soil after the end of the growing season