With the ultimate aim of improving fixation of Ti implants,The the specific the affinity of phosphorylated peptides for TiO2 has beenis well established and is applied widewidely applied ly (12). Previously, we showed thedemonstrated that binding occurs between TiO2 and phosphoproteins, such as caseins, phosvitin, and bone phosphoproteins (2 – 4). However, as far as we know, there has been no systematic study of the interaction between TiO2 and collagen. Therefore, iIn this study, we analyzedH heere we report an investigation of the the behavior of soluble collagen using column chromatography in which on the column chromatographyusing columns packed with 45mm TiO2 beads. In addition, (45 mm). And we have studied the interaction between the TiO2 (anatase) powders and aggregating collagen and . Also, we observed the attachment of anatase powders and Ti-bead s on the surface of to collagen fibers