Macrophage and neutrophil response

Macrophage and neutrophil response to parasite infection in vivoTo analyze Toxoplasma-macrophage interactions over time, 3dpf transgenic larvae possessing red macrophages Tg(mpeg1:Gal4-FF)gl25/Tg(UAS-E1b:nfsB.mCherry)c264 (herein referred to as mpeg1:G/U:mCherry), were infected with type I, II or III Toxoplasma-GFP, and macrophage recruitment was quantified by fluorescent stereomicroscopy. As compared to mock injection, the number of macrophages recruited to the infection site is significantly increased (~1.5 fold) for all three strain types at both 6 and 24hpi (Fig. 3A).To analyze Toxoplasma-neutrophil interactions over time, 3dpf transgenic larvae possessing red neutrophils Tg(lyz:dsRed)nz50 (herein referred to as lyz:dsRed), were infected with type I, II or III Toxoplasma-GFP, and neutrophil recruitment was quantified by fluorescent stereomicroscopy. Here, the number of neutrophils recruited to the infection site is significantly increased (~3 fold) as compared to mock injection for all three strain types at 6hpi (Fig. 3B). In contrast to macrophages, which remain at the infection site by 24hpi, the number of neutrophils recruited to the infection site (for all three strain types) is significantly decreased to basal levels by 24hpi.