The principle of the chromatographic column is that when the mobile phase containing the sample passes through the stationary phase, because the stationary phase has different passage resistances for different chemical substances, the time it takes for different chemical substances to flow from the inlet to the outlet is different, thereby separating the different chemical substances. The degree of separation, peak time, and symmetry of different chemical substances are affected by mobile phase composition, column temperature, pre-column pressure, etc. The best test conditions can be obtained by adjusting various influencing factors.
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