氨苄抗性的琼脂平板在37℃培养12小时后，观察平板菌落生长情况。（2）

After culturing the ampicillin-resistant agar plate at 37°C for 12 hours, observe the growth of colonies on the plate. <br>(2) Pick a single colony into 2ml of ampicillin-resistant LB culture medium, shake the bacteria overnight at 37°C. <br>(3) Select the turbid LB culture medium for bacterial liquid PCR, refer to the PCR reaction system and reaction parameters in step 2.2.1.1, and then identify the target fragment by agarose nucleic acid electrophoresis.

Ampicillin-resistant agar plates were cultured at 37 degrees C for 12 hours to observe the growth of plate bacterios.<br>(2) Pick a single bacteria to fall into 2 ml of ampicillin-resistant LB culture, 37 degrees C shaker overnight.<br>(3) Select the turbid LB culture liquid to do the bacteria liquid PCR, refer to the PCR reaction system and reaction parameters in step 2.2.1.1, and then the agar glycoic acid electrophoresis identification purpose fragments.

After the ampicillin resistant agar plates were cultured at 37 ℃ for 12 hours, the colony growth was observed.<br>(2) Select a single colony into 2ml ampicillin resistant LB medium, shake the bacteria at 37 ℃ overnight.<br>(3) The turbid LB culture medium was selected for PCR. The PCR reaction system and reaction parameters in step 2.2.1.1 were referred, and the target fragment was identified by agarose nucleic acid electrophoresis.<br>