Figure 3-4 Silibinio affected mitoc

Figure 3-4 Silibinio affected mitochondrial function via p53 and JNK. Mitochondrial membrane potential （MMP, △Ψm） was measured by Rhodamine 123 staining. Con: control. Sili: silibinin. PFT-a: pifithrin-a. SP: SP600125. A: The cells were treated with different concentration of silibinin. Then MMP was detected by a flow cytometry staining with Rhodamine 123. B: The cells were pretreated with pifithrin-a fbr 1 h, and cultured with silibinin fbr 12 h. Then MMP was detected by a flow cytometry staining with Rhodan ine 123. C: The cells were pretreated vith SP600125 fbr 1 h, and cultured with silibinin fbr 12 h. Then MMP was detected by a flow cytometry stained with Rhodamine 123.