[Objective] To study the influence of lactic acid bacteria, mixed bacteria of lactic acid bacteria and yeast, and Aspergillus niger on the quality of quinoa straw fermented feed, to provide a reference for the implementation and development of quinoa straw in feed. 【Method】 Use microbial fermentation to improve the nutritional value of quinoa straw. In the single fermentation of lactic acid bacteria, set three test factors: fermentation time, water content and inoculum addition amount. In the test of fermented quinoa straw mixed with lactic acid bacteria and yeast, set three test factors of fermentation time, water content and mixing ratio. All experiments were designed using the orthogonal principle of L9 (34), and the four nutrition indicators of crude fiber, crude protein, crude fat and soluble sugar were treated with response surface method. At the same time, a simple comparative test of Aspergillus niger on quinoa straw fermented feed was conducted. [Results] 1) Under the same fermentation conditions, the effect of the mixed preparation on degrading cellulose is more obvious than that of a single starter; 2) The average protein content of quinoa straw feed after fermentation increased by 2.7% compared with that of unfermented quinoa straw; 3 ) The change of crude fat in the fermentation process is small, between 0.24% and 0.31%: 4) After fermentation with the starter, the average soluble sugar content of quinoa straw feed increased by about 3%; 5) Aspergillus niger has obvious The degradation of cellulose and the increase of soluble sugar content, but the sensory quality of Aspergillus niger fermented feed is very poor. [Conclusion] Microbial fermentation has a certain positive effect on feed quality. Under certain conditions, cooperative fermentation is better than single fermentation.
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