abstractIn the later period of female koala taking care of her young.her body consumption gradually increases due to the joey’s growth and feeding, and her nutritional supply can not keep up with her, It can lead to weight loss and other problems of mother koala, and even lead to diseases. In this paper, taking the mother koala Fluffy and Flower as the research objects, we tried to separate the cubs from the mother in advance. Through comparative analysis of the baby's weight, behavior, mother koala weight, and body condition, the results showed that this separation method had no adverse effect on the growth and development of Koala cubs, and at the same time improved the body condition of female koala, which provided reference for the koala breeding. Key words: koala, Cub separation, body weight