Adjust the scale on the image to match the length of the physical scale ruler You can move the scale or alter the length using the touch function. In the example shown, a scale with a fine division of 0.01 mm (10 µm) is used. The total length is 1000 µm Enter an appropriate name for the calibration. Usually the lens magnification set for the microscope is selected. In the example here: 4x. It is also vital to specify the length of the part as established using the scale, as well as the preferred measuring unit for the measurement. In the example here: 1000 µm.Click on “Calculate” in order to save and close the calibration. This procedure must be carried out again for other lens magnifications.