The magnitude of potential carryove

The magnitude of potential carryover was not calculated； but was judged to be acceptable based on chromatographic results. Mean precision and accuracy at the LLOQ (n=3) ranged from 7.1 to 13.1% and from 91.0 to 105.3% (intra-day) and from 11.6 to 96.0%, respectively (inter-day, n=18). Mean inter-day precision and accuracy (n=18) for QCs ranged from 3.4 to 8.5% and from 94.0 to 104.9%, respectively. Mean intra-day precision and accuracy ranged from 2.3 to 10.2% and from 88.0 to 108.4%, respectively. Samples were stable for 5 F/T cycles, and for 12 h on wet ice. Matrix stability was demonstrated for 4 h at 15 to 25°C, and processed sample stability and reinjection reproducibility were acceptable after 104 h at 2 to 8°C. Samples were stable for up to 196 days frozen at -60 to -80°C. The maximum batch size was 96 injections.