CCK8 or AO/EB straining was used to

CCK8或AO / EB应变用于评估DFL或OEC在不同表面上的增殖。用蒸馏水轻轻冲洗样品。在添加DFL或OEC之前，将这些样品用完整的生长培养基（含10％胎牛血清，100 mg / mL链霉素和100 U / mL青霉素的DMEM）预润湿。然后，将2×104 DFL或OEC接种到培养皿上，或将每个Ti样品置于新的12孔板中，并保持在37.8°C的5％CO2培养箱中。在9天培养期的每一天之后，更换培养基，并将DFLs细胞与计数试​​剂一起孵育3小时。通过在450nm的波长下测量培养物中甲maz染料产物的吸光度（OD）来确定相对细胞数。为了直观地确定每个样本的OEC数量，培养3天和6天后，将含100μg/ mL AO和100μg/ mL EB（AO / EB，Sigma，美国）的双荧光染色溶液（10μL）添加到每个孔中。5分钟后，在荧光显微镜下观察所有样品。

CCK8 或 AO/EB 应变用于评估不同表面上的 DLL 或 OEC 的扩散。样品用蒸馏水轻轻冲洗。这些样品在加入DFL或OECs之前，用完整的生长介质（DMEM与10%胎儿牛血清、100mg/mL链霉素和100 U/mL青霉素）预湿。然后，2 × 104 DLL 或 OEC 被播种到放在新 12 井板中的盘子或每个 Ti 样品上，并在 37.8 °C 下保持在 5% 的 CO2 培养箱中。 在9天培养期的每一天之后，培养本都改变，用计数试剂孵育DSL细胞3小时。通过测量450 nm波长培养物中阿马赞染料产品的光吸收度（OD）确定相对细胞数。为了直观地确定每个样品的OEC数量，在培养3天和6天后，将含有100μg/mL AO和100μg/mL EB（AO/EB，美国西格玛）的双荧光染色溶液（10 μL）添加到每个井中。5分钟后，所有样品在荧光显微镜下观察。

CCK8 or AO/EB straining was used to evaluate the proliferation of the DFLs or OECs on different surfaces. The samples were gently rinsed with distilled water. These samples were prewetted with a complete growth medium (DMEM with 10% fetal bovine serum, 100 mg/mL streptomycin, and 100 U/mL penicillin) before adding DFLs or OECs. Then, 2 × 104 DFLs or OECs were seeded onto a dish or each Ti sample placed in a new 12-well plate and were maintained in a 5% CO2 incubator at 37.8 °C. After each day of the 9-day culture period, the medium was changed, and the DFLs cells were incubated with the counting reagent for 3h. The relative cell number was determined by measuring the light absorbance (OD) of the formazan dye product in the cultures at a wavelength of 450 nm. To visually determine the number of OECs for each sample, dual fluorescence staining solution (10 μL) containing 100 μg/mL AO and 100 μg/ mL EB (AO/EB, Sigma, USA) was added to each well after 3 and 6 days of culture. After 5 minutes, all the samples were viewed under fluorescence microscope.<br>