辣子草水浸提液处理组的黏胶层增厚幅度最大，根边缘细胞凋亡率最小，相反，

The thickening of the viscose layer was the largest in the treatment group of the spicy grass water extract, and the apoptosis rate of the root edge cells was the smallest. Spicy grass can stimulate the marginal cells of tartary buckwheat root to secrete a large amount of protective mucus to relieve the damage of allelopathy. The water extract of the schizonepeta is highly toxic to the buckwheat root edge cells, which affects the intracellular mucus secretion system, resulting in the root edge cells secreting insufficient viscous layer to resist allelopathy in the water extract, resulting in cell withering Die.

Spicy grass water immersion liquid treatment group of the largest thickening of the mucosa layer, root edge apoptosis rate is the smallest, on the contrary, the smallest change in the soil jing mustard water leachate treatment group, the root edge apoptosis rate is the largest, indicating that the spicy grass can stimulate the bitter buckwheat root edge cells secreted a large number of protective mucus relief substances to its damage. The soil mustard water-soaked liquid is highly toxic to the edge cell of buckwheat root, affecting the cell mucus secretion system, resulting in the mucous layer secreted by the root edge cells is not sufficient to resist the chemical substance in the water-soaked liquid, which leads to apoptosis.

In the treatment group of horseradish water extract, the thickening range of mucilage layer was the largest, and the apoptosis rate of root marginal cells was the smallest. On the contrary, in the treatment group of soil wattle mustard water extract, the change range was the smallest, and the apoptosis rate of root marginal cells was the largest, which indicated that horseradish could stimulate buckwheat root marginal cells to secrete a lot of protective mucus to alleviate the damage of allelochemicals. The toxicity of the aqueous extract of Schizonepeta tenuifolia to the limbic cells of buckwheat roots was strong, which affected the secretion system of intracellular mucus, resulting in that the mucilage layer secreted by the limbic cells of buckwheat roots was not enough to resist the allelochemicals in the aqueous extract, thus leading to apoptosis.