Inoculated in conventional AGAR tilting medium and iron trisaccharide medium (TSI) for preliminary biochemical identification; Pure culture showed that the response to iron trisaccharide medium was very consistent with the suspected growth of escherichia coli. Normally, e. coli EHEC does not ferment sorbitol, so much so that it appears as a colorless colony on a medium containing sorbitol, while other e. coli appear pink.