Growth identified as Bacillus was o

Growth identified as Bacillus was observed on blood agar plates streaked with fluid from the non-viable negative control article injected egg.The death of this embryo likely resulted from the bacterial contamination.The yolk sac material from all viable eggs in each group was pooled.A 10% suspension of pooled yolk sac material was injected into ten new seven day old embryonated eggs using the same route of injection.All of the embryos were viable.No evidence of viral contamination was observed due to the test article, SB-MCB-ACC-454-100 12 Feb 96 31 Jul 2000.The test will be considered valid if ninety percent of the control adult mice, eighty percent of the control suckling mice, eighty percent of the control embryonated hen's eggs, and seventy-five percent of the control guinea pigs survive the observation period, show no lesions at the site of injection or signs of viral infection.There may be instances when the test article animals meet the evaluation criteria, but the negative control animals do not meet the criteria detailed above, yet the assay will be considered valid.