Determination of MIC of Hordenine. The chemicalstructure of hordenine is presented in Figure 1A. The MIC ofhordenine was evaluated by doubling dilution assay with concentrations ranging from 0.625 to 10 mg mL−1. The MIC ofhordenine was 2.5 mg mL−1. The growth profile was determined using hordenine at sub-MIC concentrations for 24 h(Figure 1B). Treatment with hordenine at concentrations varying from 0.5 to 1.0 mg mL−1 showed no inhibitory effect on cellgrowth compared with the control.