Additionally, the spheroids can be

Additionally, the spheroids can be implanted in vivo and used in animal models or within specialized techniques such as the dorsal skin fold window chamber, creating an in vitro/in vivo assay. For decades, it has been postulated that growing tumor cell-only spheroids and implanting them in animals lead to a much higher tumor take with far fewer total cells inoculated. This was hypothesized to be due to the creation of a microenvironment in the spheroid as well as possibly engaging or enriching for cancer stem cell phenotype. Although the optimal conditions vary when co-culturing different cell types with EC, when using tumor-EC spheroids, as few as two spheroids per implantation are sufficient to generate tumor growth and metastasis [164]. This approach may be valuable in studying various treatments or aspects of tumor progression thought to be dependent on vascularization and/or stimulation of metastasis or aggressiveness via tumor cells engaging EC.

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结果 (简体中文) 1: [复制]

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另外，可将球体植入体内并用于动物模型中或在 专门技术（例如背侧皮肤折叠窗小室）内使用，以进行体外/ 体内测定。几十年来，已经假定生长仅肿瘤细胞的球体并将其 植入动物体内会导致更高的肿瘤发生率，而 接种的总细胞却少得多。据推测，这是由于在 球体中形成了微环境，以及可能参与或富集了癌症干细胞表型。尽管 将不同细胞类型与EC共培养时的最佳条件有所不同，但是当使用 肿瘤EC球体时，每次植入少至两个球体就足以产生 肿瘤的生长和转移[164]。该方法在研究 被认为依赖于 通过参与EC的肿瘤细胞的血管化和/或刺激转移或侵袭性的肿瘤进展的各种治疗或方面中可能是有价值的。

正在翻译中..

结果 (简体中文) 2:[复制]

复制成功！

Additionally, the spheroids can be implanted in vivo and used in animal models or within specialized techniques such as the dorsal skin fold window chamber, creating an in vitro/in vivo assay. For decades, it has been postulated that growing tumor cell-only spheroids and implanting them in animals lead to a much higher tumor take with far fewer total cells inoculated. This was hypothesized to be due to the creation of a microenvironment in the spheroid as well as possibly engaging or enriching for cancer stem cell phenotype. Although the optimal conditions vary when co-culturing different cell types with EC, when using tumor-EC spheroids, as few as two spheroids per implantation are sufficient to generate tumor growth and metastasis [164]. This approach may be valuable in studying various treatments or aspects of tumor progression thought to be dependent on vascularization and/or stimulation of metastasis or aggressiveness via tumor cells engaging EC.

正在翻译中..

结果 (简体中文) 3:[复制]

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此外，球体可以植入体内，用于动物模型或体内 专门技术，如背部皮肤折叠窗室，创造一个体外/内 活体分析。几十年来，人们一直认为生长中的肿瘤细胞只有球体和 把它们植入动物体内会导致肿瘤的发生率更高，而总细胞却少得多 接种了疫苗。假设这是由于 球体也可能参与或丰富肿瘤干细胞表型。尽管 不同细胞类型与EC共培养的最佳条件不同 肿瘤EC球体，每次植入只要两个球体就足以产生 肿瘤生长和转移[164]。这种方法在研究 肿瘤进展的治疗或方面被认为依赖于血管化 和/或通过肿瘤细胞与EC结合刺激转移或侵袭性。

正在翻译中..

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