There are five soil washouts indica

There are five soil washouts indicated as Falcons S1-S5. There are also five leaf extracts (microtubes L1-S5).Note:The 5 soil washouts (S1-S5) correspond to the 5 leaf extracts (L1-L5), one by one (S1belongs to L1). But we do not yet know which of these these soil washout and leaf extract pairs belong to which plant (Plants A-E).By performing protocols 2.1 to 2.3, measure the CaCO3 and Nitrate concentration of soil washout samples and the pH of both soils washouts and plant leaf extracts.Note: The leaf extracts were prepared at dawn.Protocol 2.1. Measuring the soil CaCO content To investigate the total concentration of all salts in soil, we measure CaCO3 content of soil washouts byconducting volumetric assay. “Solution 1 for CaCO3 measurement” contains an indicator that turns blue when there is no free Ca2+ in the mixture. “Solution 2 for CaCO3 measurement” contains compound X (4mM) that forms 1:1 complex with Ca2+ ions.1. Add 10 mL of the soil washout to the beaker. (Wash the beaker with distilled water before this step, if necessary).2. Add 2 drops of the “Solution 1 for CaCO measurement” and mix. If the solution is blue, the CaCO3 concentration is ≤1mg/L. If the solution is red, then proceed to next step3. Using syringe, add “CaCO3 solution 2” to the mixture until the colour turns blue. Shake the beaker while adding the solution. Write the volume of “Solution 2 for CaCO measurement”” consumed and Ca2+ concentration of soil washouts in Question 2: Table 1 of the answer sheet (Ca molar mass = 40g/mole).Protocol 2.2: Measuring the Nitrate content To measure Nitrate content of soil washouts, we use a kit. Instructions of the kit are as follow:1. Pour 1 mL of the soil washout sample in the 25mL or 50mL beaker.2. Add 20 drops of the “Solution 1 for NO measurement” carefully and then add distilled water until the total volume reaches 10 mL.3. Carefully and using gloves and tweezers, pick 2 KOH pellets and dissolve them in the above mixture.4. By adding drops of “Solution 2 for NO measurement”, a yellow colour will appear.5. Continue to add “Solution 2 for NO measurement” drop-wise and shake until the yellow colour is stable.6. Transfer about 5 mL of the mixture to a test tube and compare its color with the color reference strip forNO measurement. Record the result in Question 2: Table 1 of the answer sheet.