Staining: At desired time points, the cultures were fixed with4 vol.% paraformaldehyde (Sigma-Aldrich), permeabilized with 0.1 vol.%Triton X-100 (Thermo Fisher), and blocked using 5 wt.% goat serum(Sigma-Aldrich). F-actin was stained with rhodamine-phalloidin (ThermoFisher) for 20 min at room temperature and nuclei were counterstainedwith 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher) for 5 min.