As chloroplast-specific SSRs are uniparentally inherited and are inclined to undergo slipped-strand mispairing, they are often used in population genetics, species identification, and evolutionary process research of wild plants [38,39]. In addition, chloroplast genome sequences are highly conserved,and the SSR primer for chloroplast genomes can be transferred across species and genera. In this study , The types, presence, and distribution of SSRs in the chloroplast genome of A. Venetum were studied. a total of 273 SSRs were found in A. Venetum, most of them distributed in LSC and SSC and partly distributed in IR. These included 105 mononucleotide SSRs (38.46%) ,142 dinucleotide SSRs (50.01%), 10 trinucleotides, 14 tetranucleotides, and 2 pentanucleotides repeats, and the mononucleotide A and T repeat units accounted for the largest portion.