Resuspend the pellet in 0,7m1 AIM V culture medium and process the T-SPOT.TBAssay according the procedure for the PBMCs.Remark: Cell counting should be carried out using a hemocytometer rather than an automatedhemoanalyzer since there are likely to be a high concentration of macrophages which may giveerroneous results when a hematology ana加zer is used. Even when using a hemocytometer it may be difficult to count the cells due to the high concentration of macrophages therefore only the smaller, viable cells should be counted,. Similarly the suspension should be pipetted from the centre of the well rather than close to the wall of the well to reduce the number of macrophages obtained..When a black" cell suspension is incubated over night the samples should be wash 6一8 times to eliminate the black" macrophages.