Living tracking of rounding CTCs or elongated CTCs in same zebrafish brain capillaries in kdrl:eGFP Casper embryo. Imaris Surface analysis (Top view and Lateral view) of the CTCs were used to determine the shape of the CTCs; white arrows indicating round CTCs (6h panel) or extravasated CTCs (22h panel), yellow arrows indicating elongated CTCs. (e) Imaris Surface analysis of both CTCs (red) and capillaries (green) distinguish the intravascular and extravasated CTCs (white arrows)