开展几种盐生植物和甜土植物液泡膜Na+/H+逆向转运蛋白生物信息学分析

Several carried Halophytes soil and plants sweet tonoplast Na + / H + antiporter bioinformatic analysis showed the presence of sweet plants and soil Halophyte N- glycosylation sites, phosphorylation sites and tertiary structure significant differences, especially multiple alignment amino acid analysis showed the difference more amino acid sites may lead to sweet soil and plant functional differences halophytes key sites. <br>2. First product to carry out a salt-type Halophytes King and sweet soil plant Vacuolar Na + / H + antiporter of yeast functional complementation experiments, the results show the ability of the mutant strain of yeast into salt ZxNHX1 gene and stronger than the transfer AtNHX1 AtNHX2 mutant strain of yeast. <br>3. Were extracted from the expression product salt type Halophyte King vacuolar membrane Na + / H + antiporter gene encoding ZxNHX1, sweet soil plant Arabidopsis thaliana vacuolar membrane Na + / H + complete bubble antiporter gene encoding AtNHX1 and AtNHX2 mutant yeast strain using acridine oRANGE labeled fluorescence quenching method were determined ZxNHX1, AtNHX1 AtNHX6 protein expression and ion exchange active, Na + transport activity was found: ZxNHX1> AtNHX2> AtNHX1; K + transport activity: AtNHX1> AtNHX2> ZxNHX1

The bioinformatics analysis of several salt-growing plants and sweet-soil plant liquid foam membranes, Na-H-and-H-and-Tsing-intranste proteins, showed that there were obvious differences between the N-glycobase sites, phosphorylation sites and the tertiary structures of sweet soil plants and salt plants, especially the analysis of amino acid multiple-comparison sites, which may have led to the differences between the functions of sweet and salt plants.<br>2. For the first time, the complementary experiments of salt-forming salt-growing plant bullking and sweet soil plant amoeba foam membrane Na/H-reverse-transshipment protein yeast were carried out, and the results showed that the salt resistance of the yeast mutation strain transferred to the ZxNHX1 gene was stronger than that of the yeast mutation strain transferred to AtNHX1 and AtNHX2.<br>3. The complete liquid bubbles of the expression salt-salt-type plant-dominant liquid bubble membrane ZxNHX1, the sweet soil plant amoeba liquid bubble film Na and H and the reverse transport protein coding gene AtNHX1 and AtNHX2 yeast mutant strains are extracted respectively, and the fluorescence of the slug orange label is quenched by the radon orange marker fluorescence The ion exchange activity of ZxNHX1, AtNHX1 and AtNHX2 expression proteins was measured, and the activity of Na-transshipment was found: ZxNHX-AtNHX1;

Bioinformatics analysis of Na + / H + antiporter in vacuoles of several halophytes and sweet earth plants was carried out. The results showed that there were significant differences in N-glycosylation sites, phosphorylation sites and tertiary structures between sweet earth plants and salt plants, especially in amino acid multiple comparison analysis. The differences of multiple amino acid sites may be the key to the functional differences between sweet earth plants and salt plants Locus.<br>2. For the first time, we carried out the function complementary experiment of Na + / H + antiporter in vacuole membrane of halophyte overlord and sweet soil plant Arabidopsis thaliana. The results showed that the salt tolerance ability of yeast mutant with zxnhx1 gene was stronger than that of yeast mutant with atnhx1 and atnhx2 gene.<br>3. Extraction and expression of Na + / H + antiporter gene in vacuole membrane of overlord The Na + / H + antiporter gene atnhx1 and atnhx2 of vacuolar membrane of zxnhx1 and Arabidopsis thaliana were identified by using acridine orange labeled fluorescence quenching method