According to the Laviron method for a surface-controlled electrochemical system, the apparent heterogeneous electron transfer rate constant (ks) of tyrosinase immobilized on Tyr-MXene-Chi/GC was estimated to be about 1.62 s−1, which was higher than that 0.66 s−1 for tyrosinase immobilized on graphene choline-gold electrode and 1.15 ± 0.04 s−1 for Tyr/NiO nanoparticles modified GC electrode, suggesting a faster electron-transfer process.