The amount of live yeast was determined by directcounting in a Burker chamber. The samples offermented wine were diluted at ratios of 1:10 and1:100 and were treated with methylene blue solutionfor easy differentiation of live and dead cells. Thesamples were pippetted onto a clean glass slide in theBurker chamber and placed under the microscope(Olympus CX 31) at 40x magnification. Seventy twofields were monitored and evaluated (Babikova,2010). Determination was carried out immediately intriplicate in 20 mL samples, which were taken beforemicro-oxygenation, after micro-oxygenation, and atthe end of fermentation.