A comparison between the values from micro-DSC curves and the flexpoint temperature of the sigmoids from DPHs fluorescence anisotropy obtained for the modelmembrane alone and containing the 20% of various FFAs is reported in Table 2. We observe that theflex point temperatures obtained from the spectroscopic experiments are in substantial accordancewith the values of the calorimetric ones, confirming once again the type of structural effectproduced by FFAs with different molecular geometry well reflects the effects on the membranethermodynamic stability (slight differences may be ascribable to the lower sensitivity of thespectroscopic technique and to the few points obtained by the discrete heating ramp with 3°C steps).Conclusively, the peculiar effects of FFAs on the phospholipid bilayer of the model system,based on their chemical structure, can be considered as reference for the detection of the possibleFFA-nisin combined action on the model membrane in each case.