Image correlation coefficients comp

Image correlation coefficients comparing time points show a strong positive correlation(0.55-0.75)whencomparedtothecoefficientsgenerated by comparing randomly different locations (?0.05 to +0.05)(Figures 1B andS1C). While at greater depths in the lower dermis we noticed some movement of nuclei, the movement was comparable to that obtained at the same depth when remounting and reimaging the paw within a few minutes of the first image (Figure 1B). Altogether, these data show that fibroblast position is remarkably stable in unperturbed non-hairy skin.