酪氨酸酶（EC1.14.18.1）是一种多功能的、活性中心含有两个Cu

Tyrosinase (EC1.14.18.1) is a multifunctional metal oxidase with two Cu2+ active centers, also known as polyphenol oxidase, which is widely present in animals, plants, microorganisms and humans. Tyrosinase plays an important role as a key rate-limiting enzyme in the synthesis of melanin in organisms. Melanin is the main cause of melanosis in aquatic products, black spots in mammals and enzymatic browning of fruits and vegetables. Therefore, it is of great significance to establish a scientific and effective method for the detection of tyrosinase content in aquatic products, fruits and vegetables, and provide a new reference standard for realizing the quality evaluation of aquatic products, fruits and vegetables. At present, people have established a variety of method systems for the detection of tyrosinase activity, which are mainly divided into three categories: colorimetry, electrochemistry and fluorescence. These methods have certain advantages in terms of operability, sensitivity, and accuracy. Among them, electrochemical method is a more ideal method, which has outstanding advantages in terms of automation degree, detection speed, and real-time monitoring of complex systems.

Tyrosinease (EC1.14.18.1) is a versatile, active center containing two Cu2 plus metal oxidase, also known as polyphenol oxidase, widely found in plants and animals, microorganisms and the human body. Tyrosine enzymes play an important role as key speed limit enzymes in the synthesis of melanin in organisms. Melanin is the main cause of blackness in aquatic products, black spots in mammals and browning in fruits and vegetables. Therefore, it is of great significance to establish a scientific and effective method for detecting tyrosine enzyme content in aquatic products and fruits and vegetables, and to provide a new reference standard for the quality evaluation of aquatic products and fruits and vegetables. At present, people have established a variety of methods for the detection of tyrosine enzyme activity, mainly divided into three categories: color method, electrochemical method and fluorescence method. These methods have certain advantages in operability, sensitivity and accuracy. Among them, electrochemical method is a more ideal method, in the degree of automation and detection speed and real-time monitoring of complex systems and other advantages are more prominent.

Tyrosinase (ec1.14.18.1) is a multi-functional metal oxidase with two Cu2 + in its active center, also known as polyphenol oxidase, which widely exists in animals, plants, microorganisms and human body. Tyrosinase, as a key rate limiting enzyme, plays an important role in melanin synthesis. Melanin is the main cause of blackening of aquatic products, black spots of mammals and enzymatic browning of fruits and vegetables. Therefore, it is of great significance to establish a scientific and effective method for the detection of tyrosinase content in aquatic products and fruits and vegetables, and provide a new reference standard for the quality evaluation of aquatic products and fruits and vegetables. At present, people have established a variety of methods for the detection of tyrosinase activity, mainly divided into three categories: colorimetry, electrochemical method and fluorescence method. These methods have some advantages in operability, sensitivity and accuracy. Among them, electrochemical method is a more ideal method, which has outstanding advantages in automation, detection speed and real-time monitoring of complex systems.<br>